Background: Studies have demonstrated that inflammation has a key role in the pathogenesis of atherosclerosis\r\ndue to the abnormal gene expressions of multiple cytokines. We established an accurate and precise method to\r\nobserve gene expression in whole blood that might provide specific diagnostic information for coronary artery\r\ndisease (CAD) and other related diseases.\r\nMethods: The fifteen selected CAD-related genes (IL1B, IL6, IL8, IFNG, MCP-1, VWF, MTHFR, SELL, TNFalpha,\r\nubiquitin, MCSF, ICAM1, ID2, HMOX1 and LDLR) and two housekeeping genes (ACTB and GK) as internal references\r\nhave been measured simultaneously with a newly developed multiplex polymerase chain reaction (multi-PCR)\r\nmethod. Moreover, the precision was evaluated, and a procedure for distinguishing patients from the normal\r\npopulation has been developed based upon analyses of peripheral blood. A total of 148 subjects were divided into\r\ngroup A (control group without plaques), group B (calcified plaques) and group C (non-calcified plaques, and\r\ncombination group) according dual-source CT criteria. Gene expression in blood was analyzed by multi-PCR, and\r\nlevels of glucose and lipids measured in 50 subjects to explore the relationship among them.\r\nResults: The precision results of the multi-PCR system revealed within-run and between-run CV values of\r\n3.695ââ?¬â??12.537% and 4.405ââ?¬â??13.405%, respectively. The profiles of cytokine gene expression in peripheral blood were\r\nset: a positive correlation between glucose and MCSF, HMOX1 or TNFalpha were found. We also found that\r\ntriglyceride levels were negatively correlated with SELL gene expression in 50 subjects. Compared with controls,\r\ngene expression levels of IL1B, IL6, IL8 and MCP-1 increased significantly in group C.\r\nConclusions: A new multiple gene expression analysis system has been developed. The primary data suggested that\r\ngene expression was related to CAD. This system might be used for risk assessment of CVDs and other related diseases.
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